Leica SP5 User Guide

The Leica Sp5 is an inverted laser-scanning confocal microscope with a 405 nm laser diode, a white-light laser (WLL), and four PMT (photo-multiplier tube) detectors. The system is enclosed with an incubation unit to regulate experimental temperature and air gas (5% CO2) for live cell imaging.

The objective turret houses five lenses including 10x Air, 20x Air, 40x Oil, 63x Water, 63x Oil, and 100x Oil immersion. The stage is motorized for large tiled stitching or marking and storing positions.

For access please follow the Request Usage directions.

The objectives available for use on the SP5 are: