Microscopes

Nikon A1R   

•Confocal •Laser Scanning •2-Photon •4 Visible Lasers •1k Resonant Scanner •Upright Microscope Configuration   

Nikon C2 Macro Confocal

•Laser Scanning •Large Area Scanning •4 Visible Lasers •High Acquisition Automation •Upright Microscope Configuration

Leica SP5 

•Confocal •Laser Scanning •Tunable Filters •Environmental Control •5 PMTs •8 Laser Lines 

Leica SP8 - PodB Stem Cell Building

•Confocal •Laser Scanning •Tunable Filters •White Light Laser •2 PMTs •2 HyD Detectors  •Navigator Module

Leica SP5 - PodD Stem Cell Building

•Confocal •Laser Scanning •Tunable Filters •Inverted Configuration •4 PMTs •White Light Laser •Environmental Chamber

Leica Stellaris 5 - PodA Stem Cell Building

•Confocal •Laser Scanning •Tunable Filters •Inverted Configuration •4 Laser Lines •Navigator Module

Zeiss Widefield - "ZipScope" & "Wide Guy"   

•Widefield •Sutter Lambda XL •High Acquisition Automation •µMagellan  •Inverted Microscope Configuration • Live Cell Imaging      

Zeiss Apotome

•Optical Sectioning  •Automated Structed Illumination •Upright Microscope Configuration 

Gen3 Multiphoton 

•Laser Scanning •2-Photon •6 Channel Detection •2 IR Lasers •High Acquisition Automation •µMagellan •Upright Microscope Configuration

Lattice Light Sheet Microscope - "LLS"   

• Bessel Beam Lattice •3 Visible Lasers •Fast 3D Volumes •Low Phototoxicity •Richardson-Lucy Deconvolution  

 
Nikon SoRa Spinning Disk Microscope

•Spinning Disk Confocal •4 Visible Lasers •Super-Resolution •Environmental Chamber •High Acquisition Automation

Nikon Spinning Disk - Wittman Lab   

•Confocal •Spinning Disk •4 Visible Lasers •High Acquisition Automation •Inverted Microscope Configuration    

Leica SP8

•Confocal •Laser Scanning •Tunable Filters •White Light Laser •3 PMTs •2 HyD Detectors •Lightning Deconvolution •Navigator Module

Leica SP5 - PodC Stem Cell Building

•Confocal •Laser Scanning •Tunable Filters •Upright Configuration •4 PMTs •9 Laser Lines

Leica Thunder Imager - PodB Stem Cell Building

•Computational Clearing Widefield •Environmental Chamber •Inverted Configuration •LED Illumination •Fast Acquisition

Keyence - PodC Stem Cell Building

•Microscope in a Box •4 Channel Fluorescence •Brightfield •Enclosed System •Slide Scanning •Z-Stack

Zeiss Total Internal Reflection Fluorescence - "TIRF"   

•TIRF •4 TIRF Lasers •Lambda DG4 •Brightfield •DualView System •Widefield Option •IRM Ready   

Zeiss Brightfield   

•Brightfield •AmScope Camera •AmScope Software •High Magnification Objectives •Upright Configuration 

Gen4 Multiphoton  

•Laser Scanning •2-Photon •6 Channel Detection •2 IR Lasers •High Acquisition Automation •µMagellan •Upright Microscope Configuration    

Selective Plane Illumination Microscope - "SPIM"   

•Fast Volume Acquisition •3 Visible Lasers •Cleared Tissues •Deconvolution •µMagellan  


 Support Instruments

Caliper IVIS Spectrum   

•In vivo Luminescence Imager •Part of the Anatomy Recharge Unit

Leica Vibratome  

•Automated Vibratome •Live Tissue Sectioning •Fixed Tissue Sectioning   

3D Printer  

•Form2 •SLA UV Curing •Spot Size:140µm •Maximum Build Size:145 x 145 x 175mm 

 Precisionary Compresstome VF-310-0Z

•Automated Vibratome •Fragile Tissues •Samples are Embedded in Agarose  

Pipette Puller    

•Automatic •Sutter Instruments 

Alveole PRIMO Micropatterning System

•1-2µm resolution •1second full field pattern •Auto Alignment


 Analysis Stations

Analysis Station 1 (Linux System)   

•128 GB RAM •32 Cores •8GB GeForce RTX 2080 •Pop!_OS 19.04

Analysis Station 2  

•128 GB RAM •Intel Core i9 •PNY Quadro P4000 •Imaris •Matlab •Zen Blue

 Analysis Station 3  

•128 GB RAM •Intel Core i9 •PNY Quadro P4000 •Imaris •Matlab •Zen Blue   

Analysis Station 4 (Development Station)

•64 GB RAM •Intel i7 •Nvidia GeForce 780 •Imaris •Matlab •Zen Blue


 Image Analysis

Consultation   

•Appointments •Collaboration   

Code Respository    

•GitHub •Matlab •Python •Macros 


 Other Microscopy Resources


Microscope Maintenace