Microscopes

Nikon A1R   

•Confocal •Laser Scanning •2-Photon •4 Visible Lasers •High Acquisition Automation •Upright Microscope Configuration    

Nikon C1si

•Confocal •Laser Scanning •4 Visible Lasers •Spectral Detection •Automated Z Acquistion •Upright Microscope Configuration    

Nikon C2 Macroconfocal Confocal

•Laser Scanning •Large Area Scanning •4 Visible Lasers •High Acquisition Automation •Upright Microscope Configuration   

Nikon Spinning Disk - Wittman Lab   

•Confocal •Spinning Disk •4 Visible Lasers •High Acquisition Automation •Inverted Microscope Configuration     

Zeiss Spinning Disk - "Werb Scope"   

•Confocal •Spinning Disk •4 Visible Lasers •High Acquisition Automation •MicroManager  •µMagellan  •Inverted Microscope Configuration    

Zeiss Widefield - "Wide Guy"   

•Widefield •Sutter Lambda XL •High Acquisition Automation •µMagellan  •Inverted Microscope Configuration • Live Cell Imaging      

Zeiss Apotome

•Optical Sectioning  •Automated Structed Illumination •Upright Microscope Configuration          

Zeiss Brightfield   

•Brightfield •Axiovision

Zeiss Total Internal Reflection Fluorescence - "TIRF"   

•TIRF •2 TIRF Lasers •Lambda DG4 •Brightfield •DualView System •IRM Ready   

Leica SP5 

•Confocal •Laser Scanning •Tunable Filters •Environmental Control •5 PMTs •8 Laser Lines   

Leica SP8

•Confocal •Laser Scanning •Tunable Filters •White Light Laser •3 PMTs •2 HyD Detectors •Lightning Deconvolution

Gen3  

•Laser Scanning •2-Photon •6 Channel Detection •2 IR Lasers •High Acquisition Automation •µMagellan •Upright Microscope Configuration    

Gen4   

•Laser Scanning •2-Photon •6 Channel Detection •2 IR Lasers •High Acquisition Automation •µMagellan •Upright Microscope Configuration    

Lattice Light Sheet Microscope - "LLS"   

• Bessel Beam Lattice •3 Visible Lasers •Fast 3D Volumes •Low Phototoxicity •Richardson-Lucy Deconvolution   

Selective Plane Illumination Microscope - "SPIM"   

•Fast Volume Acquisition •3 Visible Lasers •Cleared Tissues •Deconvolution •µMagellan  


 Support Instruments

Caliper IVIS Spectrum   

•In vivo Luminescence Imager •Part of the Anatomy Recharge Unit

Leica Vibratome  

•Automated Vibratome •Live Tissue Sectioning •Fixed Tissue Sectioning   

3D Printer  

•Form2 •SLA UV Curing •Spot Size:140µm •Maximum Build Size:145 x 145 x 175mm 

 Home Built Vibratome

•Manual Vibratome •Fragile Tissues •Samples are Embedded in Agarose   

Pipette Puller    

•Automatic •Sutter Instruments 

Alveole PRIMO Micropatterning System

•1-2µm resolution •1second full field pattern •Auto Alignment


 Analysis Stations

Analysis Station 1 (Linux System)   

•128 GB RAM •32 Cores •8GB GeForce RTX 2080 •Pop!_OS 19.04

Analysis Station 2  

•128 GB RAM •Intel Core i9 •PNY Quadro P4000 •Imaris •Matlab •Zen Blue

 Analysis Station 3  

•128 GB RAM •Intel Core i9 •PNY Quadro P4000 •Imaris •Matlab •Zen Blue   

Analysis Station 4 (Development Station)

•64 GB RAM •Intel i7 •Nvidia GeForce 780 •Imaris •Matlab •Zen Blue


 Image Analysis

Consultation   

•Appointments •Collaboration   

Code Respository    

•GitHub •Matlab •Python •Macros 


 Other Microscopy Resources


Microscope Maintenace