Microscopes
Nikon A1R
•Confocal •Laser Scanning •2-Photon •4 Visible Lasers •1k Resonant Scanner •Upright Microscope Configuration
Nikon C2 Macro Confocal
•Laser Scanning •Large Area Scanning •4 Visible Lasers •High Acquisition Automation •Upright Microscope Configuration
Leica SP5
•Confocal •Laser Scanning •Tunable Filters •Environmental Control •5 PMTs •8 Laser Lines
Leica SP8 - PodB Stem Cell Building
•Confocal •Laser Scanning •Tunable Filters •White Light Laser •2 PMTs •2 HyD Detectors •Navigator Module
Leica SP5 - PodD Stem Cell Building
•Confocal •Laser Scanning •Tunable Filters •Inverted Configuration •4 PMTs •White Light Laser •Environmental Chamber
Leica Stellaris 5 - PodA Stem Cell Building
•Confocal •Laser Scanning •Tunable Filters •Inverted Configuration •4 Laser Lines •Navigator Module
Zeiss Widefield - "ZipScope" & "Wide Guy"
•Widefield •Sutter Lambda XL •High Acquisition Automation •µMagellan •Inverted Microscope Configuration • Live Cell Imaging
Zeiss Apotome
•Optical Sectioning •Automated Structed Illumination •Upright Microscope Configuration
Gen3 Multiphoton
•Laser Scanning •2-Photon •6 Channel Detection •2 IR Lasers •High Acquisition Automation •µMagellan •Upright Microscope Configuration
Lattice Light Sheet Microscope - "LLS"
• Bessel Beam Lattice •3 Visible Lasers •Fast 3D Volumes •Low Phototoxicity •Richardson-Lucy Deconvolution
Nikon SoRa Spinning Disk Microscope
•Spinning Disk Confocal •4 Visible Lasers •Super-Resolution •Environmental Chamber •High Acquisition Automation
Nikon Spinning Disk - Wittman Lab
•Confocal •Spinning Disk •4 Visible Lasers •High Acquisition Automation •Inverted Microscope Configuration
Leica SP8
•Confocal •Laser Scanning •Tunable Filters •White Light Laser •3 PMTs •2 HyD Detectors •Lightning Deconvolution •Navigator Module
Leica SP5 - PodC Stem Cell Building
•Confocal •Laser Scanning •Tunable Filters •Upright Configuration •4 PMTs •9 Laser Lines
Leica Thunder Imager - PodB Stem Cell Building
•Computational Clearing Widefield •Environmental Chamber •Inverted Configuration •LED Illumination •Fast Acquisition
Keyence - PodC Stem Cell Building
•Microscope in a Box •4 Channel Fluorescence •Brightfield •Enclosed System •Slide Scanning •Z-Stack
Zeiss Total Internal Reflection Fluorescence - "TIRF"
•TIRF •4 TIRF Lasers •Lambda DG4 •Brightfield •DualView System •Widefield Option •IRM Ready
Zeiss Brightfield
•Brightfield •AmScope Camera •AmScope Software •High Magnification Objectives •Upright Configuration
Gen4 Multiphoton
•Laser Scanning •2-Photon •6 Channel Detection •2 IR Lasers •High Acquisition Automation •µMagellan •Upright Microscope Configuration
Selective Plane Illumination Microscope - "SPIM"
•Fast Volume Acquisition •3 Visible Lasers •Cleared Tissues •Deconvolution •µMagellan
Support Instruments
Caliper IVIS Spectrum
•In vivo Luminescence Imager •Part of the Anatomy Recharge Unit
Leica Vibratome
•Automated Vibratome •Live Tissue Sectioning •Fixed Tissue Sectioning
3D Printer
•Form2 •SLA UV Curing •Spot Size:140µm •Maximum Build Size:145 x 145 x 175mm
Precisionary Compresstome VF-310-0Z
•Automated Vibratome •Fragile Tissues •Samples are Embedded in Agarose
Pipette Puller
•Automatic •Sutter Instruments
Alveole PRIMO Micropatterning System
•1-2µm resolution •1second full field pattern •Auto Alignment
Analysis Stations
Analysis Station 1 (Linux System)
•128 GB RAM •32 Cores •8GB GeForce RTX 2080 •Pop!_OS 19.04
Analysis Station 2
•128 GB RAM •Intel Core i9 •PNY Quadro P4000 •Imaris •Matlab •Zen Blue
Analysis Station 3
•128 GB RAM •Intel Core i9 •PNY Quadro P4000 •Imaris •Matlab •Zen Blue
Analysis Station 4 (Development Station)
•64 GB RAM •Intel i7 •Nvidia GeForce 780 •Imaris •Matlab •Zen Blue
Image Analysis
Consultation
•Appointments •Collaboration
Code Respository
•GitHub •Matlab •Python •Macros
Other Microscopy Resources
Microscope Maintenace