Total Internal Reflection Fluorescence
Major Funding Provided By:
Krummel Lab

Mouse OT1 Tcells. Image Credit: En Cai, PhD. Krummel Lab

TIRF User Guide

Our through-the-objective TIRF microscope is based on an inverted Zeiss Axioscope body utilizing many of the automated components and a Dual-View system for simultaneous imaging. The system runs on both Micro-Manager and Metamorph. Laser lines available for imaging with TIRF include 405nm, 488nm, 561nm and 642nm. Additional imaging modalities include IRM and brightfield.

For access please follow the Request Usage directions.

The objectives available for use on the TIRF are: