Colloquially known as "Werb Scope", the microscope is a Yokogawa style spinning disk confocal on a Zeiss Axiovert 200M base. Compared to a traditional laser scanning confocal microscope that uses a single pinhole to direct light onto a sample and then to a PMT, a spinning disk microscope uses a rotating array of microlenses and pinholes to excite many sample location, which are then projected upon a camera. Advantages of this design include increased light throughput (attributed to the microlenses) and decreased image acquisition time (attributed to the camera).
Werb Scope uses a 4-line (405nm, 488nm, 560nm, 640nm) fiber optic laser launch for excitation. The Andor Clara High Resolution camera is used for image collection.
For access please follow the Request Usage directions.
The objectives available for use on the Werb Scope are:
